Publications

Subdiffraction fluorescence lifetime imaging with Flipper-TR

Julia Rubio-Loscertales, Jose Requejo-Isidro, Cristina Flors

Methods and Applications in Fluorescence, 2026, 14, 047001

A FAIR road to a bioAFM databank

Nature Methods, 2026, 23, 1927

Exploring bacteria–surface interactions with a fluorescent membrane tension probe

Carmen Gonzalez-Garcia, Daniel Ballesteros, Jaime J. Hernandez, Manuel Pazos, Isabel Rodríguez, Jose Requejo-Isidro, Cristina Flors

PNAS, 2025, 122, e2512977122

How much force is needed to kill a single bacterium?

Virginia Vadillo-Rodríguez, Patricia Pedraz, Cristina Flors

Small, 2025, 21 2407990

In Situ Single-Cell Bacterial Imaging Provides Mechanistic Insight into the Photodynamic Action of Photosensitizer-Loaded Hydrogels

Ingrid V. Ortega, Tuğçe Şener Raman, Agnes Schulze, Cristina Flors

ACS Applied Materials and Interfaces, 2024, 16, 5, 5677

Min oscillations in bacteria as real-time reporter of environmental challenges at the single-cell level

Ingrid V. Ortega, Felipe Viela, Cristina Flors

Open Biology, 2023, 13: 230020

Real-Time Imaging of the Mechanobactericidal Action of Colloidal Nanomaterials and Nanostructured Topographies

Felipe Viela, Ingrid V. Ortega, Jaime J. Hernández, Isabel Rodríguez, Sara Moreno-Da Silva, Alejandro López-Moreno, Emilio M. Pérez, Cristina Flors

Small Science, 2023, 2300002

Activatable iodinated BODIPYs for selective imaging and photodynamic disruption of amyloid structures in pathogenic bacterial biofilms

J. Torra, T. Sawazaki, P. Bondia, S. Nonell, M. Kanai, Y. Sohma, C. Flors

Sensors and Actuators B: Chemical, 2022, 371, 132475

Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2

J. Torra, F. Viela, D. Megias, B. Sot, C. Flors

Chemistry- A European Journal, 2022, 28, e202200026

Min Oscillations as Real-time Reporter of Sublethal Effects in Photodynamic Treatment of Bacteria

I. V. Ortega, J. Torra, C. Flors

ACS Infectious Diseases, 2022, 8, 86

Boosting the inactivation of bacterial biofilms by photodynamic targeting of matrix structures with Thioflavin T

P. Bondia, C. Flors, J. Torra

Chemical Communications, 2021,57, 8648

9-Aryl-phenalenones: Bioinspired thermally reversible photochromic compounds for photoswitching applications in the pico-to milliseconds range

R. Bresolí-Obach, W. A. Massad, A. Abudulimu, L. Lüer, C. Flors, J. G. Luis, L. I. Rosquete, Teresa A. Grillo, O. Anamimoghadam, G. Bucher, S. Nonell

Dyes and Pigments, 2021,186, 109060

Long-term STED imaging of amyloid fibers with exchangeable Thioflavin T

J. Torra, P. Bondia, S. Gutierrez-Erlandsson, B. Sot, C. Flors

Nanoscale, 2020,12, 15050-15053

Mechanically Induced Bacterial Death Imaged in Real Time: A Simultaneous Nanoindentation and Fluorescence Microscopy Study

A. del Valle, J. Torra, P. Bondia, C. M. Tone, P. Pedraz, V. Vadillo-Rodríguez, C. Flors

ACS Applied Materials & Interfaces 2020, 12 (28), 31235–31241

Nanoscale view of amyloid photodynamic damage

P. Bondia, J. Torra, C. M. Tone, T. Sawazaki, A. del Valle, B. Sot, S. Nonell, M. Kanai, Y. Sohma, C. Flors

J. Am Chem. Soc. 2020, 142, 922

Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein

Joaquim Torra, Céline Lafaye, Luca Signor, Sylvain Aumonier, Cristina Flors, Xiaokun Shu, Santi Nonell, Guillaume Gotthard, Antoine Royant

Sci. Rep. 2019, 9, 2428

Linear assembly of lead bromide-based nanoparticles inside lead (II) polymers prepared by mixing the precursors of both the nanoparticle and the polymer

S. Gonzalez-Carrero, L. Bareño, E. Debroye, C. Martin, P. Bondia, C. Flors, R. E Galian, J. Hofkens, J. Pérez-Prieto

Chem. Commun. 2019, 55, 2968-2971

Mechanics of Virus-like Particles Labeled with Green Fluorescent Protein

Johann Mertens, Patricia Bondia, CarolinaAllende-Ballestero, José L. Carrascosa, Cristina Flors, José R. Castón

Biophys. J. 2018, 115(8),1561

Singlet Oxygen: Chemistry, Applications and Challenges Ahead

Cristina Flors, Axel G. Griesbeck, Georgios Vassilikogiannakis

ChemPhotoChem 2018, 2(7) 510

Fluorescent Flavoprotein Heterodimers: Combining Photostability with Singlet Oxygen Generation

Alberto Rodríguez‐Pulido, Joaquim Torra, Sara H. Mejías, Aitziber L. Cortajarena, Rubén Ruiz‐González , Santi Nonell, Cristina Flors

ChemPhotoChem 2018, 2(7) 571

Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples

P. Bondia,S. Casado, C. Flors

Methods in Molecular Biology 2017, 1663, 105-113

Super-resolution Fluorescence Imaging for Materials Science

D. Wöll, C. Flors

Small Methods 2017, 1(10), 1700191

Hybrid nanoscopy of hybrid nanomaterials

P. Bondia, R. Jurado, S. Casado, J. M. Domínguez-Vera, N. Gálvez, C. Flors

Small 2017, 13(17), 1604128

Photoswitching-Enabled Contrast Enhancement in Light Sheet Fluorescence Microscopy

T. Vettenburg, A. Corral, A. Rodríguez-Pulido, C. Flors, J. Ripoll

ACS Photonics 2017, 4(3), 424-428

Real-time imaging of photodynamic action in bacteria

A. Gollmer, A. Felgentraeger, T. Maisch, C. Flors

J. Biophoton. 2017, 10(2), 264-270

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

A. Rodríguez-Pulido, A. L. Cortajarena, J. Torra, R. Ruiz-González, S. Nonell and C. Flors

Chem. Commun., 2016, 52, 8405-8408

Apoferritin Fibers: a New Template for 1D Fluorescent Hybrid Nanostructures

R. Jurado, F. Castello, P. Bondia, S. Casado, C. Flors, R. Cuesta, J. M. Domínguez-Vera, Á. Orte and N. Gálvez

Nanoscale 2016, 8, 9648-9656

Antibacterial Activity of DNA-Stabilized Silver Nanoclusters Tuned by Oligonucleotide Sequence

S. Javani, R. Lorca, A. Latorre, C. Flors, A. L. Cortajarena, and Á. Somoza

ACS Appl. Mater. Interfaces 2016, 8 (16), pp 10147–10154

Biologically controlled synthesis and assembly of magnetite nanoparticles

M. Bennet, L. Bertinetti, R. K. Neely, A. Schertel, A. Körnig, C. Flors, F. D. Müller, D. Schüler, S. Klumpp, D. Faivre

Faraday Discuss 2015, 18, 71-83.

β-Phenyl quenching of 9-phenylphenalenones: a novel photocyclisation reaction with biological implications

G. Bucher, R. Bresolí-Obach, C. Brosa, C. Flors, J. G. Luis, T. A. Grillo, S. Nonell

Phys. Chem. Chem. Phys. 2014,16, 18813-18820

Correlative Atomic Force Microscopy and Localization-Based Super-Resolution Microscopy: Revealing Labelling and Image Reconstruction Artefacts

A. Monserrate, S. Casado, C. Flors

ChemPhysChem 2014, 5, 647–650

Singlet Oxygen Generation by the Genetically Encoded Tag miniSOG

R. Ruiz-González, A. L. Cortajarena, S. H. Mejias, M. Agut, S. Nonell, C. Flors

J. Am. Chem. Soc., 2013, 135 (26), 9564–9567

Super-resolution fluorescence imaging of directly labelled DNA: from microscopy standards to living cells

C. Flors

J. Microscopy 2013, 251, 1-4

Naphthoxanthenyl, a New Stable Phenalenyl Type Radical Stabilized by Electronic Effects

O. Anamimoghadam, M. D. Symes, C. Busche, D.-L. Long, S. T. Caldwell, C. Flors, S. Nonell, L. Cronin, G. Bucher

Org. Lett., 2013, 15 (12), 2970–2973

Single-molecule imaging at high hydrostatic pressure

H. Vass, S. L. Black, C. Flors, D. Lloyd, F. B. Ward, R. J. Allen

Appl. Phys. Lett. 2013, 102, 154103

Fluorescent proteins as singlet oxygen photosensitizers: mechanistic studies in photodynamic inactivation of bacteria

R. Ruiz-González, J. H. White, A. L. Cortajarena, M. Agut, S. Nonell, C. Flors

Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 859609 (2013)

A genetically-encoded photosensitiser demonstrates killing of bacteria by purely endogenous singlet oxygen

R. Ruiz-González, J. H. White, M. Agut, S. Nonell, C. Flors

Photochem. Photobiol. Sci., 2012,11, 1411-1413

Reversible Fluorescence Photoswitching in DNA

D. A. Smith, P. Holliger, C. Flors

J. Phys. Chem. B, 2012, 116 (34), 10290–10293

Super-resolution fluorescence microscopy as a tool to study the nanoscale organization of chromosomes

C. Flors, W. C. Earnshaw

Curr. Op. Chem. Biol, 2011, 15, 838–844

FIT for Purpose: PNA-Based Probes Enable mRNA Imaging in Living Cells

J. Tilsner, C. Flors

ChemBioChem, 2011, 12 (7) ,1007-1009

DNA and Chromatin Imaging with Super-Resolution Fluorescence Microscopy Based on Single-Molecule Localization

C. Flors

Biopolymers 2011, 95, 290-7

Quantification of Photosensitized Singlet Oxygen Production by a Fluorescent Protein

X. Ragàs, L. P. Cooper, J. H. White, S. Nonell, C. Flors

ChemPhysChem 2011, 12, 161-5

Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore

A. Jiménez-Banzo, X. Ragàs, S. Abbruzzetti, C. Viappiani, B. Campanini, C. Flors, S. Nonell

Photochem. Photobiol. Sci., 2010,9, 1336-1341

A super-resolution map of the vertebrate kinetochore

S. A. Ribeiro, P. Vagnarelli, Y. Dong, T. Hori, B. F. McEwen, T. Fukagawa, C. Flors, W. C. Earnshaw

PNAS 2010, 107, 10484–10489

Photoswitching of monomeric and dimeric DNA-intercalating cyanine dyes for super-resolution microscopy applications

C. Flors

Photochem. Photobiol. Sci., 2010,9, 643-648

Super-Resolution Imaging of DNA Labelled with Intercalating Dyes

C. Flors, C. N. J. Ravarani, D. T. F. Dryden

ChemPhysChem 2009, 10, 2201–2204

Constitutively active RhoA inhibits proliferation by retarding G(1) to S phase cell cycle progression and impairing cytokinesis

P. Morin, C. Flors, M. F. Olson

Eur. J. Cell Biol 2009, 88, 495–507

Nonlinear optical properties of photoswitchable fluorescent proteins

I. Asselberghs, C. Flors, E. De Meulenaere, B. Champagne, J. Vanderleyden, K. Clays

Proc. SPIE 7403, Nanobiosystems: Processing, Characterization, and Applications II, 74030P (2009)

Second-Harmonic Generation in GFP-like Proteins

I. Asselberghs, C. Flors, L. Ferrighi, E. Botek, B. Champagne, H. Mizuno, R. Ando, A. Miyawaki, J. Hofkens, M. Van der Auweraer, K. Clays

J. Am. Chem. Soc., 2008, 130 (46), pp 15713–15719

How is cis-trans isomerization controlled in Dronpa mutants? A replica exchange molecular dynamics study

S. L. C. Moors , S. Michielssens, C. Flors, P. Dedecker, J. Hofkens, A. Ceulemans

J. Chem. Theory Comput., 2008, 4 (6), pp 1012–1020

Dark states in monomeric red fluorescent proteins studied by fluorescence correlation and single molecule spectroscopy

J. Hendrix, C. Flors, P. Dedecker, J. Hofkens, Y. Engelborghs

Biophys. J. 2008, 94, p4103–4113

Singlet Oxygen Photosensitization by EGFP and its Chromophore HBDI

A. Jiménez-Banzo, S. Nonell, J. Hofkens, C. Flors

Biophys. J. 2008, 94, 168–172

Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: Numerical analysis and application to the fluorescent protein Dronpa

P. Dedecker , J. Hotta ,C. Flors, M. Sliwa ,H. Uji-i ,M. B. J. Roeffaers, R. Ando, H. Mizuno, A. Miyawaki, J. Hofkens

J. Am. Chem. Soc., 2007, 129 (51), pp 16132–16141

A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants

C. Flors, J. Hotta, H. Uji-i ,P. Dedecker, R. Ando, H. Mizuno, A. Miyawaki , J. Hofkens

J. Am. Chem. Soc., 2007, 129 (45), pp 13970–13977

3D nanoscopy: Bringing biological nanostructures into sharp focus

P. Dedecker, C. Flors, J. Hotta, H. Uji-i, J. Hofkens

Angewandte Chem. Int. Ed. 2007, 46, 8330–8332

Single perylene diimide dendrimers as single-photon sources

M. Sliwa, C. Flors, I. Oesterling, J. Hotta, K. Müllen, F. C. De Schryver, J. Hofkens

J. Phys.: Condens. Matter 19 445004

Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants

R. Ando, C. Flors, H. Mizuno, J. Hofkens, A. Miyawaki

Biophys. J. 2007, 92, L97–L99

Ultrafast excited-state dynamics of the photoswitchable protein dronpa

E. Fron, C. Flors, G. Schweitzer, S. Habuchi, H. Mizuno, R. Ando, F. C. De Schryver, A. Miyawaki, J. Hofkens

J. Am. Chem. Soc., 2007, 129 (16), pp 4870–4871

Energy and electron transfer in ethynylene bridged perylene diimide multichromophores

C. Flors ,I. Oesterling, T. Schnitzler, E. Fron, G. Schweitzer, M. Sliwa, A. Herrmann, M. van der Auweraer, F. C. de Schryver, K. Müllen, J. Hofkens

J. Phys. Chem. C, 2007, 111 (12), pp 4861–4870

Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching

S. Habuchi, P. Dedecker, J. Hotta, C. Flors, R. Ando, H. Mizuno, A. Miyawaki, J. Hofkens

Photochem. Photobiol. Sci., 2006,5, 567-576

Photoconversion in the red fluorescent protein from the sea anemone Entacmaea quadricolor: Is cis-trans isomerization involved?

D. Loos , S. Habuchi, C. Flors, J. Hotta, J. Wiedenmann, G. U. Nienhaus, J. Hofkens

J. Am. Chem. Soc., 2006, 128 (19), pp 6270–6271

Solvent and pH dependent fluorescent properties of a dimethylaminostyryl borondipyrromethene dye in solution

M. Baruah, W. Qin, C. Flors , J. Hofkens, R. A. L. Vallée , D. Beljonne , M. Van der Auweraer, W. M. De Borggraeve, N. Boens

J. Phys. Chem. A, 2006, 110 (18), pp 5998–6009

Light and singlet oxygen in plant defense against pathogens: Phototoxic phenalenone phytoalexins

C. Flors, S. Nonell

Acc. Chem. Res., 2006, 39 (5), pp 293–300

Imaging the production of singlet oxygen in vivo using a new fluorescent sensor, Singlet Oxygen Sensor Green (R)

C. Flors, M. J Fryer, J. Waring, B. Reeder, U. Bechtold, P. M. Mullineaux, S. Nonell, M. T. Wilson, N. R Baker

J. Exp. Bot. (2006) 57 (8): 1725-1734

Phototoxic phytoalexins. Processes that compete with the photosensitized production of singlet oxygen by 9-phenylphenalenones

C. Flors, P. R. Ogilby, J. G. Luis, T. A. Grillo, L. R. Izquierdo, P-L Gentili, L. Bussotti, S. Nonell

Photochem. Photobiol. 2006, 82, 95–103

Photochemistry of phytoalexins containing phenalenone-like chromophores: Photophysics and singlet oxygen photosensitizing properties of the plant oxoaporphine alkaloid oxoglaucine

C. Flors, C. Prat, R. Suau, F. Nájera, S. Nonell

Photochem. Photobiol. 2005, 81, 120-124

Light- and singlet oxygen-mediated antifungal activity of phenylphenalenone phytoalexins

A. Lazzaro, M. Corominas, C. Martí, C. Flors, L. R. Izquierdo, T. A. Grillo, J. G. Luis, S. Nonell

Photochem. Photobiol. Sci., 2004,3, 706-710

Radical species derived from phenalenone: characterization and role of upper excited states

C. Flors, S. Nonell

J. Photochem. Photobiol. A. Chem. 2004, 163, 9-12

On the phosphorescence of 1H-phenalen-1-one

C. Flors, S. Nonell

Helv. Chim. Acta 2001, 84, 2533–2539